| 1. | She's not fond of himquite offish and quite careless .
她并不喜欢他对他相当冷淡，完全不挂在心上。
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| 2. | He ' s not like geoffrey , he seems stand - offish
他不像格雷戈里,他对我很不友好
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| 3. | Southern jiangsu is a famous land offish and rice in chinese history
苏南地区是我国历史上著名的鱼米之乡。
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| 4. | Brenda is a very friendly girl but her parents are a bit stand - offish
布伦达是一个非常友好的姑娘，可是她父母亲有点冷漠。
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| 5. | She used to be aloof and stand - offish with us , but we are now on first - name terms
她一向对我们疏远冷淡，但现在我们成了很好的朋友了。
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| 6. | Making anti - dis ease breeding researches of fish by transfer gene is a new direction in transfergene researches offish
通过基因转移进行鱼类抗病育种研究，是鱼类转基因研究中的一个新的方向。
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| 7. | The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以细胞培养液m199 (含2既的小牛血清，常规量双抗)为冻存稀释液对泥鳅胚胎细胞冷冻保存宜采取先慢后快的方式(例如，从0一一6 ，冻存速度为一0 . 05 / min ，再以一0 . 5 / min的速度从一6一一40 ) ; dmso的保护效应浓度为12 . 506左右;小牛血清的浓度对泥鳅胚胎细胞的成活率影响不明显;囊胚晚期细胞抗冻性比中早期强;通过对不同批次的冻存细胞解冻培养，解冻后成活率为30 %以上细胞培养数天后均有少数细胞贴壁，但只发现两瓶培养细胞有明显增殖现象产生许多未分化的小细胞。
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| 8. | For the cryogenic preservation of fish , in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp , the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture , the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要从鱼类种质保存的目的出发，一方面以四倍体鲫鲤鱼为材料，对四倍体鲫鲤鱼肾脏组织进行初步培养，为建立相应细胞库及下一步培养冻存的鱼类胚胎细胞奠定基础；另一方面，以鱼类组织细胞培养技术为基础，泥鳅胚胎细胞为材料，对鱼类胚胎细胞冻存培养方法进行初步研究，并应用该技术方法对草鱼早期胚胎细胞进行冻存培养实验。报告如下：本文用组织贴壁法对四倍体鲫鲤鱼肾组织进行原代、传代培养。
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