| 1. | Microsome mediated assay 微粒体间介试验
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| 2. | Role of the hepatocellular microsome cytochrome p450 of rats in regulation of body temperature 450在体温调节中作用的探讨
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| 3. | Preliminary data indicate that steviol may be activated to a mutagenic metabloite by human liver microsomes 初步数据显示,甜菊醇可能经人体的肝脏微粒体活化成引致基因突变的代谢物。
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| 4. | German standard methods for the examination of water , waste water and sludge - sub - animal testing group t - part 4 : determination of the genotoxic potential using the salmonella microsome test ames test 德国对水废水和污泥的标准检验法.亚门动物试验方法
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| 5. | While some data suggest that epoxidation may be involved in the metabolic activation of steviol , other data indicate that the active metabolite is not an epoxide . preliminary data indicate that steviol may be activated to a mutagenic metabloite by human liver microsomes 有些数据指出,甜菊醇在代谢活化的过程中,可能牵涉环氧化作用,但其他数据显示,甜菊醇的活性代谢物并不是环氧化物。
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| 6. | Now transgenic cell lines that express the protein encoded by ugt gene were broadly used instead of liver microsomes . the enzyme expressed in cell lines helps to realize the function of a specific gene and will provide direct information related to the isozyme interested ‘为解决上述问题,国际上人们己经广泛采用建立各种ugt转基因细胞系的方式试验,在明确基因构成的倩况下直接获得由特定基因所表达的ugt同工酶, fi什对性地研究该酶对药物的代谢作用。
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| 7. | Knowledge of distribution and regulation of ugts remains limited . liver microsomes is a frequently used enzyme source in in vitro study of glucuronidation , but it has the following deficient respects : 1 . liver microsome is a mixture of various enzymes , and therefore the results from microsome cannot represent the function of a individual isozyme 关于ugts的组织分布和调节这方面的知识还不多,过去常采用肝微粒体进行研究,但有以下缺陷: u )肝微粒体是多种药酶沮合的复杂体系,因而实验结果难以区分是一种同工醇的作用
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| 8. | Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus . for higher plants , however , though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes , only limited evidence is available for the presence and properties of the ip3r - like protein so far . here , using the immunological methods with an antibody raised against a mammalian inositol 1 , 4 , 5 - triphophate receptor ( type 1 ) , we found that , 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively 本实验用sds - page电泳和免疫印迹的方法,用哺乳动物大鼠三磷酸肌醇受体的多肽做抗体对类三磷酸肌醇受体蛋白鉴定,结果表明:抗体与水稻和拟南芥微粒体蛋白分子量大约为200kd的蛋白交叉反应,同时还发现在水稻微粒体蛋白62kd和拟南芥微粒体蛋白45kd处有交叉反应的蛋白条带存在,表明在植物中有类三磷酸肌醇受体蛋白的存在;用免疫胶体金方法,发现类三磷酸肌醇受体蛋白主要分布于液泡膜和细胞质膜上。
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| 9. | Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure 拟南芥表皮条用甲基紫精处理,可以引起气孔的关闭,当处理90分钟的时候,气孔的相对开度由100降到58 ,统计学分析气孔开度的变化有显著差异;甲基紫精引起气孔关闭的作用可以被肝素部分抑制,当甲基紫精和肝素同时处理90分钟的时候,气孔的相对开度由100降到92 ,统计学分析气孔开度的变化没有显著差异,说明气孔的相对开度基本上没有变化。
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| 10. | This experiment was performed using the " yingqing " peach fruit and " chunxing " strawberry to study the changes of the main physiology - biochemistry characteristics and the microsome membrane ca + - atpase activities during the fruits ripening . the effects of ethylene , trifluoperizine ( tfp ) + ethylene and verapamil ( ver ) + ethylene treatments on the above changes were discussed . the objects of the thesis may further explore the role of calcium messenger in the ethylene signal transduction , and provide some new theoretical basis for further research of the microsome membrane ca2 + - atpase during the fruits ripening 本论文以迎庆桃和春星草莓为试材,研究了两类果实采后在25和4条件下主要生理生化变化和ca ~ ( 2 + ) - atpase活性变化以及乙烯、钙调素拮抗剂tfp +乙烯和钙通道阻塞剂ver +乙烯处理对其的影响,为探讨果实成熟衰老过程中,钙信使系统在乙烯信号转导中的作用,提供一定的理论依据,并为探索ca ~ ( 2 + ) - atpase在果实衰老中的作用及作用机制,提供实验证据。
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