| 1. | They may be solubilized by detergents or some protein denaturants . 在去垢剂和蛋白质变性剂的作用下,它们可以变为可溶性的。
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| 2. | The study on unfolding features of denaturant - induced multiple - domain proteins 变性剂诱导的多结构域蛋白质变性现象的研究
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| 3. | The effects of metal ions and denaturants on the conformation and hemagglutinating activity of crotalaria mucronata lectin 金属离子和变性条件对野花生豆凝集素活性和构象的影响
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| 4. | Adequate amount of denaturant was added into dehydrated alcohol to produce denatured fuel alcohol , which further blended with gasoline to produce fuel alcoholic gasoline for vehicles 摘要将乙醇脱水加入适量变性剂形成“变性燃料乙醇” ,再和一定比例的汽油进一步混合,生产清洁燃料“车用燃料乙醇汽油” 。
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| 5. | The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times , and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant 用含有2mol l和4mol l尿素的包涵体洗涤液洗涤包涵体,在37条件下,洗去了大部分菌体蛋白及其它核酸物质。用8mol l尿素作为变性剂溶解包涵体,包涵体在8mol l尿素中的溶解性非常好。
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| 6. | After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant gst - eo protein by gst affinity columns . using the purified protein as coating antigen , an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum 使用分步透析法对变性的包涵体进行复性,将复性蛋白过gst亲和层析柱得到纯化的gst - eo融合蛋白。以gst - eo融合蛋白为诊断抗原,初步建立了用间接elisa检测猪瘟血清eo抗体的方法。
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